Cynomolgus IgE Protein (IGE-CM401)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus IgE, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Lys208-Lys429

Catalog: IGE-CM401

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Product Description Recombinant Cynomolgus IgE Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys208-Lys429.

Product Background

Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.

Product Category

Recombinant Protein

Target

IgE
Synonyms Ig epsilon chain C region;IgE

Gene/Protein ID

G8F4W7

Species

Cynomolgus

Exact Sequence

Lys208-Lys429
Molecular Weight The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus IgE, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus IgE, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
The purity of Cynomolgus IgE is greater than 95% as determined by SEC-HPLC.
Cynomolgus IgE on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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