Cynomolgus IL-10 Protein (IL1-CM110)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Cynomolgus IL-10, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 0.93 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ser19-Asn178

Catalog: IL1-CM110

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Product Description	Recombinant Cynomolgus IL-10 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ser19-Asn178.
Product Background	Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.
Product Category	Recombinant Protein

Target	IL-10
Gene/Protein ID	G7NVB0
Species	Cynomolgus
Exact Sequence	Ser19-Asn178
Molecular Weight	The protein has a predicted MW of 19.78 kDa. Due to glycosylation, the protein migrates to 20-22 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus IL-10, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 0.93 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-10, His Tag with an affinity constant of 0.93 nM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus IL-10, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
	Cynomolgus IL-10 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.