Name: Cynomolgus IL-10 Protein (IL1-CM110)
Brand: KactusBio
SKU: IL1-CM110-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Cynomolgus IL-10, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, hFc Tag with the EC50 of 80.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.93 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ser19-Asn178

Catalog #IL1-CM110

Size

100UG

500UG

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Product Description	Recombinant Cynomolgus IL-10 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ser19-Asn178.
Product Background	Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.
Product Category	Recombinant Protein / Antibody discovery

Protein	IL-10
Synonyms	Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451
Accession	G7NVB0
Species	Cynomolgus
Biotinylated	no
Amino Acid Range	Ser19-Asn178
Molecular Weight	The protein has a predicted MW of 19.78 kDa. Due to glycosylation, the protein migrates to 20-22 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus IL-10, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, hFc Tag with the EC50 of 80.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.93 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Cynomolgus IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-10, His Tag with an affinity constant of 0.93 nM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus IL-10, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, hFc Tag with the EC50 of 80.8ng/ml determined by ELISA (QC Test).
	Cynomolgus IL-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.