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Cynomolgus IL-11 Protein, Ultra Low Endotoxin (IL1-CM111-UL) Summary
Product Description |
Recombinant Cynomolgus IL-11 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Pro22-Leu199. |
Product Background |
Abstract Interleukin-11 (IL-11) is a pleiotropic cytokine that belongs to gp130 family. IL-11 and its receptor, IL-11Ra, are expressed in human cancers,human cancer cells expressed a functional IL-11Ra subunit, which triggered signal transduction either by exogenous recombinant human IL-11 or by autocrine production of IL-11 in cells cultured under hypoxic conditions. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Cynomolgus IL-11 Protein, Ultra Low Endotoxin (IL1-CM111-UL) Specifications/Details
Protein |
IL-11 |
Synonyms |
Interleukin-11;IL11;Adipogenesis inhibitory factor;AGIF;INN: Oprelvekin;Oprelvekin;IL-11Oprelvekin;interleukin 11;interleukin-11 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Pro22-Leu199 |
Molecular Weight |
The protein has a predicted MW of 20.48 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.25 nM as determined in SPR assay (Biacore T200). |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human IL-11 R alpha, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-11, His Tag with an affinity constant of 0.25 nM as determined in SPR assay (Biacore T200). |
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Cynomolgus IL-11 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human IL-11 R alpha, hFc Tag on ProA-Biosensor can bind Cynomolgus IL-11, His Tag with an affinity constant of 1.34 nM as determined in BLI assay . |
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The purity of Cynomolgus IL-11 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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