Name: Cynomolgus IL-12 Protein, Ultra Low Endotoxin (IL1-CM112-UL)
Brand: KactusBio
SKU: IL1-CM112-UL-100UG
Price: 454.0 USD
Availability: InStock

Cynomolgus IL-12 Protein, Ultra Low Endotoxin (IL1-CM112-UL)

Immobilized Cynomolgus IL-12, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.

Cynomolgus IL-12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus IL-12 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus IL-12, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ile23-Ser328(IL-12B) & Arg57-Ser253(IL-12A)

Catalog #IL1-CM112-UL

Size

100UG

500UG

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Product Description	Recombinant Cynomolgus IL-12 Protein is expressed from HEK293 with His tag at the C-Terminus of IL-12A.It contains Arg57-Ser253 (IL-12A) and Ile23-Ser328 (IL-12B).
Product Background	Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	IL-12
Synonyms	CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12
Accession	G7P6S2(IL-12B)&XP_005546300.2(IL-12A)
Species	Cynomolgus
Biotinylated	no
Amino Acid Range	Ile23-Ser328(IL-12B) & Arg57-Ser253(IL-12A)
Molecular Weight	The protein has a predicted MW of 34.76 kDa (IL-12B) and 23.64 kDa (IL-12A). Due to glycosylation, the protein migrates to 45-48 kDa (IL-12B) and 38-44 kDa (IL-12A) based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus IL-12, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Cynomolgus IL-12, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
	Cynomolgus IL-12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Cynomolgus IL-12 is greater than 95% as determined by SEC-HPLC.