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Cynomolgus IL-12B/p40/NKSF2 Protein (P40-CM112) Summary
Product Description |
Recombinant Cynomolgus IL-12B/p40/NKSF2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Ser328. |
Product Background |
Interleukin (IL)‑12B, which encodes the p40 subunit common to IL‑12 and IL‑23, as one of the genes for which expression in fibroblast‑like synoviocytes from patients with rheumatoid arthritis (RA‑FLS) is induced by DcR3.Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin‑related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor‑like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly affects cells as a ligand. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Cynomolgus IL-12B/p40/NKSF2 Protein (P40-CM112) Specifications/Details
Protein |
IL-12B/p40/NKSF2 |
Synonyms |
IL-12B; CLMF p40; IL-12 subunit p40; NKSF2; CLMF; CLMF2; NKS |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Ile23-Ser328 |
Molecular Weight |
The protein has a predicted MW of 35.86 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus IL-12B, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Cynomolgus IL-12B, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA (QC Test). |
|
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Cynomolgus IL-12B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus IL-12B is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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