Cynomolgus IL-13Ra1 Protein (ILR-CM1R1)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 21.16 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala25-Thr341
Catalog #ILR-CM1R1
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Product Description
 Recombinant Cynomolgus IL-13Ra1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala25-Thr341.
Product Background
 Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
Product Category
 Recombinant Protein
Target
 IL-13Ra1
Synonyms
 IL-13 R alpha 1; IL13R; IL13RA; CT19; IL13RA1; NR4; CD213A1
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Ala25-Thr341
Molecular Weight
 The protein has a predicted MW of 37.59 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 21.16 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus IL-13Ra1, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-13, His Tag with an affinity constant of 21.16 nM as determined in SPR assay (Biacore T200).
Cynomolgus IL-13Ra1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus IL-13Ra1 is greater than 95% as determined by SEC-HPLC.

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