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Cynomolgus IL-17A/CTLA-8 Protein (ILA-CM117) Summary
Product Description |
Recombinant Cynomolgus IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Gly24-Ala155. |
Product Background |
Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Cynomolgus IL-17A/CTLA-8 Protein (ILA-CM117) Specifications/Details
Protein |
IL-17A/CTLA-8 |
Synonyms |
CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gly24-Ala155 |
Molecular Weight |
The protein has a predicted MW of 16.21 kDa. Due to glycosylation, the protein migrates to 18-20 kDa, 25-27 kDa and 28-32 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 17.3ng/ml determined by ELISA. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Cynomolgus IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 17.3ng/ml determined by ELISA. |
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Immobilized Cynomolgus IL-17A, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. |
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Cynomolgus IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. |
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Loaded Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) on ProA-Biosensor can bind Cynomolgus IL-17A, His Tag with an affinity constant of 14.30 nM as determined in BLI assay . |
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The purity of Cynomolgus IL-17A is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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