• Express System: HEK293
  • Product Tag: C-His, C-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human IL-2, No Tag (Cat. IL2-HM001) at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-2 R beta&IL-2 R gamma, His, Flag Tag with the EC50 of 0.18ug/ml determined by ELISA.
  • Amino Acid Range: Ala27-Thr240(IL-2 R beta) & Leu23-255(IL-2 R gamma)
Catalog #ILR-CM1BG
Price
Regular price $33000
Regular price Sale price $33000
/
Size
Avantor VWR Fisher Scientific

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Cynomolgus IL-2 R beta&IL-2 R gamma Protein (ILR-CM1BG) Summary

Product Description

 Recombinant Cynomolgus IL-2 R beta&IL-2 R gamma Protein is expressed as a heterodimer of IL-2 R beta and IL-2 R gamma by co-expression in HEK293. Subunit IL-2 R beta is fused with a His tag at the C-terminus and subunit IL-2 R gamma is fused with a Flag tag at the C-terminus.It contains Ala27-Thr240 (IL-2 R beta) & Leu23-255 (IL-2 R gamma).

Product Background

 Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change.

Cynomolgus IL-2 R beta&IL-2 R gamma Protein (ILR-CM1BG) Specifications/Details

Protein
 IL-2 R beta&IL-2 R gamma
Synonyms
 IL-2RB&IL-2RG; IL-2RG; IL-2R subunit gamma; IL-2R subunit beta; IL-2RB; IL15RB; IL-2 R beta&IL-2 R gamma; IL-2R subunit β; IL-2 R β&IL-2 R gamma; IL-2Rγ; IL-2R γ; IL-2 Rγ; IL-2 R γ;IL2RB&IL2RG; IL2RG; IL2R subunit gamma; IL2R subunit beta; IL2RB; IL2R beta&IL2R gamma; IL2R subunit β; IL2R β&IL2R gamma; IL2Rγ; IL2R γ;IL-2R subunit b; IL2R subunit b; IL-2 R b&IL-2 R gamma; IL2R b&IL2R gamma; IL-2R gamma; IL2R gamma; IL-2R g; IL2R g; IL-2 Rg; IL-2 R gamma; IL-2 R g
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Ala27-Thr240(IL-2 R beta) & Leu23-255(IL-2 R gamma)
Molecular Weight
 The protein has a predicted MW of 31.57 kDa (IL-2 R beta) and 34.43 kDa (IL-2 R gamma). Due to glycosylation, the protein migrates to 42-52 kDa (IL-2 R beta) and 65-80 kDa (IL-2 R gamma) based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-2, No Tag (Cat. IL2-HM001) at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-2 R beta&IL-2 R gamma, His, Flag Tag with the EC50 of 0.18ug/ml determined by ELISA.
Product Tag
 C-His, C-Flag
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-2, No Tag (Cat. IL2-HM001) at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-2 R beta&IL-2 R gamma, His, Flag Tag with the EC50 of 0.18ug/ml determined by ELISA. Immobilized Human IL-2, No Tag (Cat. IL2-HM001) at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-2 R beta&IL-2 R gamma, His, Flag Tag with the EC50 of 0.18ug/ml determined by ELISA.
Immobilized Cynomolgus IL-2 R beta&IL-2 R gamma, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R gamma Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. Immobilized Cynomolgus IL-2 R beta&IL-2 R gamma, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R gamma Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
Cynomolgus IL-2 R beta&IL-2 R gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus IL-2 R beta&IL-2 R gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus IL-2 R beta&IL-2 R gamma is greater than 90% as determined by SEC-HPLC. The purity of Cynomolgus IL-2 R beta&IL-2 R gamma is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
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