Cynomolgus IL-22 Protein (IL2-CM122)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus IL-22, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-22R alpha 1, hFc Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test). The affinity constant of 60.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala34-Ile179

Catalog: IL2-CM122

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Product Description	Recombinant Cynomolgus IL-22 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala34-Ile179.
Product Category	Recombinant Protein

Target	IL-22
Gene/Protein ID	G7PHZ7
Species	Cynomolgus
Exact Sequence	Ala34-Ile179
Molecular Weight	The protein has a predicted MW of 17.69 kDa. Due to glycosylation, the protein migrates to 32-42 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus IL-22, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-22R alpha 1, hFc Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test). The affinity constant of 60.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-22, His Tag with an affinity constant of 60.21 nM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus IL-22, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-22R alpha 1, hFc Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test).
	The purity of Cynomolgus IL-22 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus IL-22 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.