Product Description |
Recombinant Cynomolgus IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag at the N-terminus.It contains Val22-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta). |
Product Background |
The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity. |
Product Category |
Recombinant Protein |
Target |
IL-23 alpha&IL-12 beta |
Synonyms |
IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Val22-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta) |
Molecular Weight |
The protein has a predicted MW of 19.52 kDa (IL-23 alpha) and 34.76 kDa (IL-12 beta). Due to glycosylation, the protein migrates to 20-25 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus IL-23 alpha&IL-12 beta, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). The affinity constant of 4.05 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human IL-23R, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 4.05 nM as determined in SPR assay (Biacore T200). |
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Immobilized Cynomolgus IL-23 alpha&IL-12 beta, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). |
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Cynomolgus IL-23 alpha&IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC. |
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