- Express System: HEK293
- Product Tag: N-His
- Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
- Activity: Immobilized Cynomolgus IL-23 alpha&IL-12 beta, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Val22-Pro189(IL-23 alpha) & Pro23-Leu328(IL-12 beta)
Product Description |
Recombinant Cynomolgus IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag at the N-terminus.It contains Val22-Pro189(IL-23 alpha) & Pro23-Leu328(IL-12 beta). |
Product Background |
The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity. |
Product Category |
Recombinant Protein |
Target |
IL-23 alpha&IL-12 beta |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Val22-Pro189(IL-23 alpha) & Pro23-Leu328(IL-12 beta) |
Molecular Weight |
The protein has a predicted MW of 19.52 kDa (IL-23 alpha) and 34.76 kDa (IL-12 beta). Due to glycosylation, the protein migrates to 20-25 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus IL-23 alpha&IL-12 beta, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Related Products
Recently viewed