Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein (IL2-CM1AB)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 57.5ng/ml determined by ELISA (QC Test). The affinity constant of 3.90 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Val22-Pro189(Cynomolgus IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta)

Catalog #IL2-CM1AB

Price

$520³⁰

Size

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Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein (IL2-CM1AB) Summary

Product Description	Recombinant Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein is expressed from HEK293 with His tag at the N-Terminus of IL-23 alpha.It contains Val22-Pro189(Cynomolgus IL-23 alpha) and Met23-Ser335(Mouse IL-12 beta).
Product Background	Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.

Protein	IL-23 alpha&IL-12 beta
Synonyms	IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β;IL12 b; IL12b; IL23 alpha&IL12 b; IL23 alpha&IL12b
Accession	A0A2K5TLV4-1(Cynomolgus IL-23 alpha)&P43432-1(Mouse IL-12 beta)
Species	Cynomolgus&Mouse
Biotinylated	no
Amino Acid Range	Val22-Pro189(Cynomolgus IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta)
Molecular Weight	The protein has a predicted MW of 19.5 (Cynomolgus IL-23 alpha)&35.8 (Mouse IL-12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Cynomolgus IL23 alpha)&40-50 (Mouse IL12 beta) kDa based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 57.5ng/ml determined by ELISA (QC Test). The affinity constant of 3.90 nM as determined in SPR assay (Biacore T200).
Product Tag	N-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Cynomolgus IL-23R, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag with an affinity constant of 3.90 nM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 57.5ng/ml determined by ELISA (QC Test).
	Immobilized Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Risankizumab, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA.
	Cynomolgus IL-23 alpha&Mouse IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Cynomolgus IL-23 alpha&Mouse IL-12 beta is greater than 95% as determined by SEC-HPLC.