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Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein (IL2-CM1AB) Summary
Product Description |
Recombinant Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein is expressed from HEK293 with His tag at the N-Terminus of IL-23 alpha.It contains Val22-Pro189(Cynomolgus IL-23 alpha) and Met23-Ser335(Mouse IL-12 beta). |
Product Background |
Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein (IL2-CM1AB) Specifications/Details
Protein |
IL-23 alpha&IL-12 beta |
Synonyms |
IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β |
Accession |
|
Species |
Cynomolgus&Mouse |
Biotinylated |
no |
Amino Acid Range |
Val22-Pro189(Cynomolgus IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta) |
Molecular Weight |
The protein has a predicted MW of 19.5 (Cynomolgus IL-23 alpha)&35.8 (Mouse IL-12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Cynomolgus IL23 alpha)&40-50 (Mouse IL12 beta) kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 57.5ng/ml determined by ELISA (QC Test). The affinity constant of 3.90 nM as determined in SPR assay (Biacore T200). |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Cynomolgus IL-23R, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag with an affinity constant of 3.90 nM as determined in SPR assay (Biacore T200). |
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Immobilized Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 57.5ng/ml determined by ELISA (QC Test). |
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Immobilized Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Risankizumab, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. |
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Cynomolgus IL-23 alpha&Mouse IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus IL-23 alpha&Mouse IL-12 beta is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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