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Cynomolgus IL-25/IL-17E Protein (IL2-CM125) Summary
Product Description |
Recombinant Cynomolgus IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-terminus.It contains Tyr33-Gly176. |
Product Background |
IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Cynomolgus IL-25/IL-17E Protein (IL2-CM125) Specifications/Details
Protein |
IL-25/IL-17E |
Synonyms |
IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Tyr33-Gly176 |
Molecular Weight |
The protein has a predicted MW of 18.18 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus IL-25, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-25 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4). |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Cynomolgus IL-25, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-25 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. |
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Cynomolgus IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus IL-25 is greater than 90% and the molecular weight of this protein is around 50-70 kDa as determined by SEC-MALS. |
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The purity of Cynomolgus IL-25 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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