Name: Cynomolgus IL-31 Protein (IL3-CM131)
Brand: KactusBio
SKU: IL3-CM131-100UG
Price: 379.5 USD
Availability: InStock

Express System: HEK293
Product Tag: N-His
Purity: >95% as determined by Bis-Tris PAGE
Activity: Immobilized Cynomolgus IL-31, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 0.59 ug/ml determined by ELISA (QC Test). The affinity constant of 1.32 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Leu27-Thr163

Catalog #IL3-CM131

Size

100UG

500UG

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Product Description	Recombinant Cynomolgus IL-31 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu27-Thr163.
Product Background	Many autoimmune skin diseases, such as bullous pemphigoid (BP), psoriasis and certain types of chronic urticaria, are associated with intensive pruritus. While histamine and neuropeptides have previously been ascribed to play a role in itch that accompanies these diseases, recent evidence suggests that the pruritogenic cytokine interleukin (IL)-31 is a major driver of pruritic responses. IL-31 was originally shown to be produced by activated helper T cells, particularly Th2 cells, mast cells, macrophages and dendritic cells.
Product Category	Recombinant Protein / Antibody discovery

Protein	IL-31
Synonyms	Interleukin-31; IL-31;IL31
Accession	G7PJ78-1
Species	Cynomolgus
Biotinylated	no
Amino Acid Range	Leu27-Thr163
Molecular Weight	The protein has a predicted MW of 16.5 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus IL-31, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 0.59 ug/ml determined by ELISA (QC Test). The affinity constant of 1.32 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	>95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-31 RA, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-31, His Tag with an affinity constant of 1.32 nM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus IL-31, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 0.59 ug/ml determined by ELISA (QC Test).
	Cynomolgus IL-31 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.