Cynomolgus IL-4 Protein (IL4-CM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus IL-4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). The affinity constant of 13.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: His25-Ser153

Catalog: IL4-CM101

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Product Description	Recombinant Cynomolgus IL-4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His25-Ser153.
Product Background	Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.-.
Product Category	Recombinant Protein

Target	IL-4
Gene/Protein ID	P79339-1
Species	Cynomolgus
Exact Sequence	His25-Ser153
Molecular Weight	The protein has a predicted MW of 16.03 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus IL-4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). The affinity constant of 13.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus IL-4, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-4 R alpha, His Tag with an affinity constant of 13.48 nM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus IL-4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).
	The purity of Cynomolgus IL-4 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus IL-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.