Product Description |
Recombinant Cynomolgus IL-4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His25-Ser153. |
Product Background |
Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.-. |
Product Category |
Recombinant Protein |
Target |
IL-4 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
His25-Ser153 |
Molecular Weight |
The protein has a predicted MW of 16.03 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus IL-4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). The affinity constant of 13.48 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Cynomolgus IL-4, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-4 R alpha, His Tag with an affinity constant of 13.48 nM as determined in SPR assay (Biacore T200). |
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Immobilized Cynomolgus IL-4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). |
|
|
Cynomolgus IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Cynomolgus IL-4 is greater than 95% as determined by SEC-HPLC. |
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