Cynomolgus IL-4 R alpha/CD124 Protein (ILA-CM14R)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human IL-4, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 97.8 ng/ml determined by ELISA (QC Test). The affinity constant of 13.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Met26-Arg232
Catalog #ILA-CM14R
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Product Description
 Recombinant Cynomolgus IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met26-Arg232.
Product Background
 Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells.
Product Category
 Recombinant Protein
Target
 IL-4 R alpha/CD124
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Met26-Arg232
Molecular Weight
 The protein has a predicted MW of 24.84 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-4, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 97.8 ng/ml determined by ELISA (QC Test). The affinity constant of 13.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus IL-4, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-4 R alpha, His Tag with an affinity constant of 13.48 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-4, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 97.8 ng/ml determined by ELISA (QC Test).
Cynomolgus IL-4 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus IL-4 R alpha is greater than 95% as determined by SEC-HPLC.

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