Cynomolgus IL-4 R alpha/CD124 Protein (ILA-CM14R)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-4, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 91.8ng/ml determined by ELISA (QC Test). The affinity constant of 13.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Met26-Arg232

Catalog: ILA-CM14R

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Product Description Recombinant Cynomolgus IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met26-Arg232.

Product Background

 Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family.The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells.

Product Category

 Recombinant Protein

Target

 IL-4 R alpha/CD124

Gene/Protein ID

 G7Q0S7

Species

 Cynomolgus

Exact Sequence

 Met26-Arg232
Molecular Weight The protein has a predicted MW of 24.84 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Human IL-4, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 91.8ng/ml determined by ELISA (QC Test). The affinity constant of 13.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus IL-4, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-4 R alpha, His Tag with an affinity constant of 13.48 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-4, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 91.8ng/ml determined by ELISA (QC Test).
The purity of Cynomolgus IL-4 R alpha is greater than 95% as determined by SEC-HPLC.
Cynomolgus IL-4 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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