Cynomolgus IL-4 R alpha/CD124 Protein (ILA-CM14R)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-4, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 91.8ng/ml determined by ELISA (QC Test). The affinity constant of 13.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Met26-Arg232

Catalog: ILA-CM14R

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Product Description Recombinant Cynomolgus IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met26-Arg232.
Product Category Recombinant Protein
Target IL-4 R alpha/CD124
Gene/Protein ID G7Q0S7
Species Cynomolgus
Exact Sequence Met26-Arg232
Molecular Weight The protein has a predicted MW of 24.84 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-4, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 91.8ng/ml determined by ELISA (QC Test). The affinity constant of 13.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus IL-4, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-4 R alpha, His Tag with an affinity constant of 13.48 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-4, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 91.8ng/ml determined by ELISA (QC Test).
The purity of Cynomolgus IL-4 R alpha is greater than 95% as determined by SEC-HPLC.
Cynomolgus IL-4 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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