Cynomolgus IL-5 Protein (IL5-CM101)
Cynomolgus IL-5 Protein (IL5-CM101)
Cynomolgus IL-5 Protein (IL5-CM101)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ile20-Ser134
Catalog #IL5-CM101
Regular price $315.00
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Product Description
Recombinant Cynomolgus IL-5 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ile20-Ser134.
Product Background
IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).
Product Category
Recombinant Protein
Target
IL-5
Synonyms
Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Ile20-Ser134
Molecular Weight
The protein has a predicted MW of 14.25 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 21.0ng/ml determined by ELISA.
Cynomolgus IL-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus IL-5 is greater than 95% as determined by SEC-HPLC.

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