• Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA). The specific activity is >200 pmol/min/ug.
  • Amino Acid Range: Ile25-Pro261
Catalog #KLK-CM003
Price
Regular price $000
Regular price Sale price $000
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Size
Avantor VWR Fisher Scientific

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Cynomolgus Kallikrein 3/PSA-Protein (active form) (KLK-CM003) Summary

Product Description

 Recombinant Cynomolgus Kallikrein 3/PSA-Protein (active form) is expressed from HEK293 without tag.It contains Ile25-Pro261.

Product Background

 Kallikrein-related peptidase 3 (KLK3), also known as prostate-specific antigen (PSA), is the most useful biomarker for prostate cancer (PCa). KLK3 is suggested to play a role in regulating cancer growth through anti-angiogenic activity in vivo and in vitro.

Cynomolgus Kallikrein 3/PSA-Protein (active form) (KLK-CM003) Specifications/Details

Protein
 Kallikrein 3/PSA
Synonyms
 PSA; Kallikrein 3; Kallikrein-3; P-30 antigen; Semenogelase; KLK3; APS;P30 antigen
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Ile25-Pro261
Molecular Weight
 The protein has a predicted MW of 26.15 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA). The specific activity is >200 pmol/min/ug.
Product Tag
 No Tag
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl, 0.05% Brij-35 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Cynomolgus Kallikrein 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus Kallikrein 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus Kallikrein 3 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus Kallikrein 3 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...