Cynomolgus KLKB1 Protein (KLK-CM1B1)
Catalog: KLK-CM1B1
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| Product Description | Recombinant Cynomolgus KLKB1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Ala638. |
Product Background |
Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides. |
Product Category |
Recombinant Protein |
Target |
KLKB1 |
Gene/Protein ID |
A0A2K5VTJ9 |
Species |
Cynomolgus |
Exact Sequence |
Gly20-Ala638 |
| Molecular Weight | The protein has a predicted MW of 70.19 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is > 800 pmol/min/ug |
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The purity of Cynomolgus KLKB1 is greater than 95% as determined by SEC-HPLC. |
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Cynomolgus KLKB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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