Product Description |
Recombinant Cynomolgus KLKB1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Ala638. |
Product Background |
Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides. |
Product Category |
Recombinant Protein |
Target |
KLKB1 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gly20-Ala638 |
Molecular Weight |
The protein has a predicted MW of 70.19 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-KLKB1 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus KLKB1, His Tag with the EC50 of 1.1ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Anti-KLKB1 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus KLKB1, His Tag with the EC50 of 1.1ug/ml determined by ELISA. |
|
|
Cynomolgus KLKB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Cynomolgus KLKB1 is greater than 95% as determined by SEC-HPLC. |
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