Cynomolgus Kremen-2 Protein (KRE-CM102)
|Recombinant Cynomolgus Kremen-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly26-Ala364.
|Kremen2 (Krm2) plays an important role in embryonic development, bone formation, and tumorigenesis as a crucial regulator of classical Wnt/β-catenin signaling pathway. Compared to para-cancerous tissues, Krm2 was significantly up-regulated in gastric cancer tissues and was positively correlated with the pathological grade of gastric cancer patients. Krm2 can be a potent candidate for designing of targeted therapy.
|The protein has a predicted MW of 37.04 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
|Immobilized Cynomolgus Kremen-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Kremen-2 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.