• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus LGR-5, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LGR-5 Antibody, hFc Avi Tag with the EC50 of 5.9ng/ml determined by ELISA.
  • Amino Acid Range: Ser22-Gly557
Catalog #LGR-CM205
Price
Regular price $345.00
Regular price Sale price $345.00
/
Size
Avantor VWR Fisher Scientific

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Cynomolgus LGR-5 Protein (LGR-CM205) Summary

Product Description

 Recombinant Cynomolgus LGR-5 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Ser22-Gly557.

Product Background

 G protein-coupled receptor 5 (LGR5), known as a stem cell marker for colon cancer and gastric cancer, can serve as a novel GSC marker involved in EMT and a therapeutic target in glioma.LGR5 is a new functional GSC marker and prognostic indicator that can promote EMT by activating the Wnt/β-catenin pathway and would thus be a novel therapeutic target for glioma.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Cynomolgus LGR-5 Protein (LGR-CM205) Specifications/Details

Protein
 LGR-5
Synonyms
 LGR5; GPR49; GPR67; HG38; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Ser22-Gly557
Molecular Weight
 The protein has a predicted MW of 84.60 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus LGR-5, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LGR-5 Antibody, hFc Avi Tag with the EC50 of 5.9ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 50mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus LGR-5, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LGR-5 Antibody, hFc Avi Tag with the EC50 of 5.9ng/ml determined by ELISA. Immobilized Cynomolgus LGR-5, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LGR-5 Antibody, hFc Avi Tag with the EC50 of 5.9ng/ml determined by ELISA.
Cynomolgus LGR-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus LGR-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus LGR-5 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus LGR-5 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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