Regular price
$346.50
Order by 4pm ET for delivery on:
Cynomolgus LILRB2/CD85d/ILT4 Protein (LIL-CM2B2) Summary
Product Description |
Recombinant Cynomolgus LILRB2/CD85d/ILT4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly24-Arg457. |
Product Background |
The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Cynomolgus LILRB2/CD85d/ILT4 Protein (LIL-CM2B2) Specifications/Details
Protein |
LILRB2/CD85d/ILT4 |
Synonyms |
CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gly24-Arg457 |
Molecular Weight |
The protein has a predicted MW of 71.68 kDa. Due to glycosylation, the protein migrates to 80-90 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 42.50 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Cynomolgus LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag (Cat. HLG-CM41CT) with an affinity constant of 42.50 nM as determined in SPR assay (Biacore T200). |
|
|
Cynomolgus LILRB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Loaded Cynomolgus LILRB2, hFc Tag on ProA-Biosensor can bind Cynomolgus HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein, His Tag (Cat. HLG-CM41CT) with an affinity constant of 41.40 nM as determined in BLI assay . |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
