Product Description |
Recombinant Cynomolgus LRG1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val72-Gln383. |
Product Background |
Diabetic nephropathy (DN) is an important public health concern of increasing proportions and the leading cause of end-stage renal disease (ESRD) in diabetic patients. It is one of the most common long-term microvascular complications of diabetes mellitus that is characterized by proteinuria and glomerular structural changes. LRG1 is a novel pro-angiogenic factors involved in the abnormal angiogenesis and renal fibrosis in DN. |
Product Category |
Recombinant Protein |
Target |
LRG1 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Val72-Gln383 |
Molecular Weight |
The protein has a predicted MW of 35.69 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus LRG1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 0.15ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Cynomolgus LRG1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 0.15ug/ml determined by ELISA. |
|
|
Cynomolgus LRG1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus LRG1 is greater than 95% as determined by SEC-HPLC. |
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