Product Description |
Recombinant Cynomolgus MADCAM1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln22-Gln295. |
Product Background |
Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) contributes to the recruitment of donor T cells into the mucosal tissues of the recipient after allogeneic hematopoietic stem cell transplantation (aHSCT). The aim of our study was to determine whether selected single nucleotide polymorphisms (SNPs) of the MADCAM1 gene are associated with development of serious complications after aHSCT. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
MADCAM1 |
Synonyms |
MAdCAM-1; MADCAM1; MACAM1 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gln22-Gln295 |
Molecular Weight |
The protein has a predicted MW of 30.65 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus MADCAM1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Cynomolgus MADCAM1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. |
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Cynomolgus MADCAM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus MADCAM1 is greater than 95% as determined by SEC-HPLC. |
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