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Cynomolgus MADCAM1 Protein, Ultra Low Endotoxin (MCM-CM101-UL) Summary
Product Description |
Recombinant Cynomolgus MADCAM1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln22-Gln295. |
Product Background |
Mucosal addressin cell adhesion molecule–1 (MAdCAM-1) is an endothelial cell marker that is recognized by its ligands on lymphocytes: L-selectin and α4β7 integrin. MAdCAM-1 is highly expressed on intestinal endothelium and has, recently, been shown to play an important role in the development of acute GVHD in animal model. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Cynomolgus MADCAM1 Protein, Ultra Low Endotoxin (MCM-CM101-UL) Specifications/Details
Protein |
MADCAM1 |
Synonyms |
MAdCAM-1; MADCAM1; MACAM1 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gln22-Gln295 |
Molecular Weight |
The protein has a predicted MW of 30.65 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus MADCAM1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Cynomolgus MADCAM1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. |
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Cynomolgus MADCAM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus MADCAM1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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