Cynomolgus MASP2 Protein (MSP-CE102)

Express System: E.coli
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Cynomolgus MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Thr287-Phe686

Catalog: MSP-CE102

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Product Description	Recombinant Cynomolgus MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Thr287-Phe686.
Product Background	The dysregulation of complement cascade leads to unsolicited cytokine storm, inflammation, deterioration of alveolar lining cells, culminating in acquired respiratory destructive syndrome (ARDS). Similar pathogenesis is observed with the middle east respiratory syndrome (MERS), severe acquired respiratory syndrome (SARS), and SARS-CoV-2. Activation of the lectin pathway via mannose-binding lectin associated serine protease 2 (MASP2) is witnessed under discrete viral infections including COVID-19.
Product Category	Recombinant Protein

Target	MASP2
Synonyms	MAP19; MASP-2; MASP1P1; sMap
Gene/Protein ID	A0A2K5UJY0
Species	Cynomolgus
Exact Sequence	Thr287-Phe686
Molecular Weight	The protein has a predicted MW of 46.8 kDa. Due to autocatalytic cleavage, the protein migrates to 21-24 kDa&28-30 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA.
	Cynomolgus MASP2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.