Product Description |
Recombinant Cynomolgus MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Thr287-Phe686. |
Product Background |
The dysregulation of complement cascade leads to unsolicited cytokine storm, inflammation, deterioration of alveolar lining cells, culminating in acquired respiratory destructive syndrome (ARDS). Similar pathogenesis is observed with the middle east respiratory syndrome (MERS), severe acquired respiratory syndrome (SARS), and SARS-CoV-2. Activation of the lectin pathway via mannose-binding lectin associated serine protease 2 (MASP2) is witnessed under discrete viral infections including COVID-19. |
Product Category |
Recombinant Protein |
Target |
MASP2 |
Synonyms |
MAP19; MASP-2; MASP1P1; sMap |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Thr287-Phe686 |
Molecular Weight |
The protein has a predicted MW of 46.8 kDa. Due to autocatalytic cleavage, the protein migrates to 21-24 kDa&28-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Cynomolgus MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. |
|
|
Cynomolgus MASP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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