Cynomolgus MASP3 Protein (MSP-CM113)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus MASP3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP3 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is > 15000 pmol/min/µg.
  • Amino Acid Range: Ile450-Ser728
Catalog #MSP-CM113
Regular price $525.00
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Size
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Cynomolgus MASP3 Protein (MSP-CM113) Summary

Product Description

Recombinant Cynomolgus MASP3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ile450-Ser728.

Product Background

Mannose-binding lectin-associated serine protease-1 (also named MASP3), a multifunctional serine protease, plays an important role in innate immunity which is capable of activating the lectin pathway of the complement system and also triggering coagulation cascade system.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Cynomolgus MASP3 Protein (MSP-CM113) Specifications/Details

Protein
MASP3
Synonyms
MASP-3; MASP-1; MASP1; RaRF; Serine protease 5; CRARF; CRARF1; PRSS5;
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Ile450-Ser728
Molecular Weight
The protein has a predicted MW of 31.94 kDa. Due to glycosylation, the protein migrates to 42-55 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus MASP3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP3 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is > 15000 pmol/min/µg.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 7.5).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus MASP3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP3 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. Immobilized Cynomolgus MASP3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP3 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
Cynomolgus MASP3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus MASP3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus MASP3 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus MASP3 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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