ELISA binding curve showing the interaction between cynomolgus MMP-9 protein and anti-MMP-9 antibody, with absorbance (OD450) plotted against the log concentration of anti-MMP-9 antibody, hFc tag
Cynomolgus MMP-9 Protein (MMP-CM109)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus MMP-9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
  • Amino Acid Range: Ala20-Asp706
Catalog #MMP-CM109
Regular price $346.50
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Size
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Cynomolgus MMP-9 Protein (MMP-CM109) Summary

Product Description

Recombinant Cynomolgus MMP-9 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala20-Asp706.

Product Background

Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.

Product Category

Recombinant Protein / Antibody discovery / ADC targets

Cynomolgus MMP-9 Protein (MMP-CM109) Specifications/Details

Protein
MMP-9
Synonyms
MMP-9; GELB; CLG4B; MANDP2; Gelatinase B
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Ala20-Asp706
Molecular Weight
The protein has a predicted MW of 77.44 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus MMP-9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus MMP-9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. Immobilized Cynomolgus MMP-9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
Cynomolgus MMP-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus MMP-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus MMP-9 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus MMP-9 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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