Cynomolgus MMP-9 Protein (MMP-CM109)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus MMP-9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala20-Asp706

Catalog: MMP-CM109

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Product Description Recombinant Cynomolgus MMP-9 Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by APMA to have hydrolytic activity.It contains Ala20-Asp706.

Product Background

Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.

Product Category

Recombinant Protein

Target

MMP-9

Gene/Protein ID

A0A2K5UU71

Species

Cynomolgus

Exact Sequence

Ala20-Asp706
Molecular Weight The protein has a predicted MW of 77.44 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus MMP-9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus MMP-9, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
The purity of Cynomolgus MMP-9 is greater than 95% as determined by SEC-HPLC.
Cynomolgus MMP-9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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