Product Description |
Recombinant Cynomolgus NGAL/Lipocalin-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Gly198. |
Product Background |
Acute kidney injury (AKI) is one of the most common complications of various serious conditions, and early diagnosis is therefore critical for the treatment of AKI. Recent evidence demonstrates that neutrophil gelatinase- associated lipocalin (NGAL) is closely associated with AKI. Several experimental and clinical studies have shown that the expression of urine and serum NGAL increases significantly in AKI. NGAL shows potential to be a new effective early biochemical marker of AKI. Further studies are needed to confirm the significant advantages of NGAL in the diagnosis of early AKI and its value in clinical applications. |
Product Category |
Recombinant Protein |
Target |
NGAL/Lipocalin-2 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gln21-Gly198 |
Molecular Weight |
The protein has a predicted MW of 21.51 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus NGAL, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-NGAL Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Cynomolgus NGAL, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-NGAL Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). |
|
|
Cynomolgus NGAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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|
The purity of Cynomolgus NGAL is greater than 95% as determined by SEC-HPLC. |
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