Cynomolgus NKG2A&CD94 Protein (NKC-CM194)
Cynomolgus NKG2A&CD94 Protein (NKC-CM194)
  • Express System: HEK293
  • Product Tag: C-His, C-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Cynomolgus NKG2A&CD94, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Pro94-Leu233(NKG2A) & Lys32-Ile179(CD94)
Catalog #NKC-CM194
Regular price $315.00
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NKC-CM194 Product Description
Recombinant Cynomolgus NKG2A&CD94 Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Pro94-Leu233(NKG2A)&Lys32-Ile179(CD94).
Product Background
The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
Product Category
Recombinant Protein
Target
NKG2A&CD94
Synonyms
CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Pro94-Leu233(NKG2A) & Lys32-Ile179(CD94)
Molecular Weight
The protein has a predicted MW of 17.01 kDa(NKG2A)&18.27 kDa(CD94). Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus NKG2A&CD94, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His, C-Flag
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus NKG2A&CD94, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
Cynomolgus NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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