Product Description |
Recombinant Cynomolgus NKG2C&CD94 Protein is expressed from HEK293 with His tag at the N-Terminus of NKG2C and Flag tag at the C-Terminus of CD94.It contains Glu98-Leu231(NKG2C)and Lys32-Ile179(CD94). |
Product Background |
NKG2C&CD94 is a C-type lectin heterodimer on NK cells and CD8+ cytotoxic T-cells, it can recognize peptides derived from the intracellular proteins in the context of HLA-E. NKG2C&CD94 itself has no signal transduction function but is an activating receptor on the surface of NK cells that involved in driving the NK-cell expansion. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
NKG2C&CD94 |
Synonyms |
NKG2C&CD94; NKG2C; CD94 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Glu98-Leu231(NKG2C) & Lys32-Ile179(CD94) |
Molecular Weight |
The protein has a predicted MW of 16.44kDa (NKG2C) & 18.27kDa (CD94). Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His, C-Flag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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