Cynomolgus NKG2D/CD314 Protein (NKG-CM12D)
Cynomolgus NKG2D/CD314 Protein (NKG-CM12D)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus NKG2D, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ile73-Val216
Catalog #NKG-CM12D
Regular price $315.00
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Product Description
Recombinant Cynomolgus NKG2D/CD314 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ile73-Val216.
Product Background
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Product Category
Recombinant Protein
Target
NKG2D/CD314
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Ile73-Val216
Molecular Weight
The protein has a predicted MW of 17.48 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus NKG2D, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus NKG2D, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA.
Cynomolgus NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus NKG2D is greater than 95% as determined by SEC-HPLC.

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