Product Description |
Recombinant Cynomolgus NKp46/NCR1/CD335 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Pro22-Asn254. |
Product Background |
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR).These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. |
Product Category |
Recombinant Protein |
Target |
NKp46/NCR1/CD335 |
Synonyms |
CD335; Ly94; NCR1; NKp46; MAR-1; NKP46FLJ99094 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Pro22-Asn254 |
Molecular Weight |
The protein has a predicted MW of 27.4 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus NKp46, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-NKp46 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl, 100mM L-arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Cynomolgus NKp46, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-NKp46 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test). |
|
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Cynomolgus NKp46 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus NKp46 is greater than 95% as determined by SEC-HPLC. |
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