• Express System: Baculovirus-Insect Cells(Sf9)
  • Product Tag: N-His
  • Purity: > 90% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
  • Activity: Immobilized Cynomolgus PADI2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-PADI2 Antibody, mFc Tag with the EC50 of 54.3ng/ml determined by ELISA.
  • Amino Acid Range: Met1-Pro665
Catalog #PAD-CB102
Price
Regular price $60000
Regular price Sale price $60000
/
Size
Avantor VWR Fisher Scientific

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Cynomolgus PADI2 Protein (PAD-CB102) Summary

Product Description

Recombinant Cynomolgus PADI2 Protein is expressed from Baculovirus-Insect Cells(Sf9) with His tag at the N-terminus.It contains Met1-Pro665.

Product Background

Peptidylarginine deiminases (PADs) are a group of enzymes that catalyze post-translational modifications of proteins by converting arginine residues into citrullines. PAD2 can be found in many tissues and organs, including brain, spinal cord, spleen , pancreas, skeletal muscles, secretory glands, and immune cells. By citrullinating proteins, PAD2 regulates a number of cellular processes such as gene transcription, antigen generation, extracellular trap formation (also termed ETosis), and pyroptosis.

Cynomolgus PADI2 Protein (PAD-CB102) Specifications/Details

Protein
PADI2
Synonyms
Protein-arginine deiminase; KIAA0994; PAD-H19; PAD2;
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Met1-Pro665
Molecular Weight
The protein has a predicted MW of 77.08 kDa. The protein migrates to 70-85 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus PADI2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-PADI2 Antibody, mFc Tag with the EC50 of 54.3ng/ml determined by ELISA.
Product Tag
N-His
Fluorophore
Unconjugated
Expression System
Baculovirus-Insect Cells(Sf9)
Purity
> 90% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS, 10% glycerol (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus PADI2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-PADI2 Antibody, mFc Tag with the EC50 of 54.3ng/ml determined by ELISA. Immobilized Cynomolgus PADI2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-PADI2 Antibody, mFc Tag with the EC50 of 54.3ng/ml determined by ELISA.
Cynomolgus PADI2 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Cynomolgus PADI2 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Cynomolgus PADI2 Protein is greater than 85% as determined by SEC-HPLC. The purity of Cynomolgus PADI2 Protein is greater than 85% as determined by SEC-HPLC.
Other Available Proteins:
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