Cynomolgus PD-L1/B7-H1 Protein (PDL-CM210)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus PD-L1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD-L1 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe19-Arg238

Catalog: PDL-CM210

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Product Description Recombinant Cynomolgus PD-L1/B7-H1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe19-Arg238.

Product Background

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Product Category

Recombinant Protein

Target

PD-L1/B7-H1
Synonyms CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Accession

Species

Cynomolgus

Exact Sequence

Phe19-Arg238
Molecular Weight The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus PD-L1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD-L1 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus PD-L1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD-L1 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.
The purity of Cynomolgus PD-L1 is greater than 95% as determined by SEC-HPLC.
Cynomolgus PD-L1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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