Cynomolgus PD-L1/B7-H1 Protein (PDL-CM210)
Cynomolgus PD-L1/B7-H1 Protein (PDL-CM210)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus PD-L1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD-L1 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Phe19-Arg238
Catalog #PDL-CM210
Regular price $150.00
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Product Description

Recombinant Cynomolgus PD-L1/B7-H1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe19-Arg238.

Product Background

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

PD-L1/B7-H1

Synonyms

CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1

Accession

Species

Cynomolgus

Biotinylated

no

Amino Acid Range

Phe19-Arg238

Molecular Weight

The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Cynomolgus PD-L1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD-L1 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Cynomolgus PD-L1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD-L1 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test). Immobilized Cynomolgus PD-L1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD-L1 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test).
Cynomolgus PD-L1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Cynomolgus PD-L1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Cynomolgus PD-L1 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus PD-L1 is greater than 95% as determined by SEC-HPLC.

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