Cynomolgus PD-L2/B7-DC Protein (PDL-CM402)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.17 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Leu20-Thr220

Catalog: PDL-CM402

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Product Description	Recombinant Cynomolgus PD-L2/B7-DC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu20-Thr220.
Product Background	PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals.
Product Category	Recombinant Protein

Target	PD-L2/B7-DC
Gene/Protein ID	XP_005581839
Species	Cynomolgus
Exact Sequence	Leu20-Thr220
Molecular Weight	The protein has a predicted MW of 23.73 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.17 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus PD-L2, His Tag with an affinity constant of 0.17 uM as determined in SPR assay (Biacore T200).
	The purity of Cynomolgus PD-L2 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus PD-L2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.