Cynomolgus PDGF R alpha/PDGFRA Protein (PFR-CM40A)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus PDGF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln24-Glu524

Catalog: PFR-CM40A

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Product Description Recombinant Cynomolgus PDGF R alpha/PDGFRA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln24-Glu524.

Product Background

 Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF.

Product Category

 Recombinant Protein

Target

 PDGF R alpha/PDGFRA

Gene/Protein ID

 Q0PW86

Species

 Cynomolgus

Exact Sequence

 Gln24-Glu524
Molecular Weight The protein has a predicted MW of 57.32 kDa. Due to glycosylation, the protein migrates to 70-85 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Cynomolgus PDGF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus PDGF R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA.
The purity of Cynomolgus PDGF R alpha is greater than 95% as determined by SEC-HPLC.
Cynomolgus PDGF R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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