Cynomolgus PLAU/uPA Protein (active form) (PLA-CM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus PLAU, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 25.1ng/ml determined by ELISA. The affinity constant of 0.033 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ser21-Leu430

Catalog: PLA-CM101

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Product Description Recombinant Cynomolgus PLAU/uPA Protein (active form) is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Leu430, which consists of two chains: Long chain A and chain B. The long chain A is further cleaved to yield a short chain A and N-Terminus fragment.

Product Background

 Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.

Product Category

 Recombinant Protein

Target

 PLAU/uPA

Gene/Protein ID

 A0A2K5WND1

Species

 Cynomolgus

Exact Sequence

 Ser21-Leu430
Molecular Weight The protein has a predicted MW of 47.23 kDa. Due to protein lysis and glycosylation, the protein migrates to 23-25 kDa (long chain A), 35-40 kDa (chain B) and 50-60 kDa (long chain A&chain B) based on Tris-Bis PAGE result.

Activity

 Immobilized Cynomolgus PLAU, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 25.1ng/ml determined by ELISA. The affinity constant of 0.033 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus uPAR, His Tag immobilized on CM5 Chip can bind Cynomolgus PLAU, His Tag with an affinity constant of 0.033 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus PLAU, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 25.1ng/ml determined by ELISA.
Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >2000 pmol/min/ug (QC Test).
The purity of Cynomolgus PLAU (active form) is greater than 95% as determined by SEC-HPLC.
Cynomolgus PLAU (active form) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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