Cynomolgus PVRIG Protein (PVR-CM401)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus PVRIG, hFc Tag at 0.1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-PVRIG Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. The affinity constant of 0.23 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Thr41-Asp171

Catalog: PVR-CM401

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Product Description Recombinant Cynomolgus PVRIG Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Thr41-Asp171 .
Product Category Recombinant Protein
Target PVRIG
Synonyms C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1
Gene/Protein ID  A0A2K5WVV8-1
Species Cynomolgus
Exact Sequence Thr41-Asp171
Molecular Weight The protein has a predicted MW of 40.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus PVRIG, hFc Tag at 0.1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-PVRIG Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. The affinity constant of 0.23 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus PVRIG, hFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-2, His Tag with an affinity constant of 0.23 μM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus PVRIG, hFc Tag at 0.1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-PVRIG Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
The purity of Cynomolgus PVRIG is greater than 95% as determined by SEC-HPLC.
Cynomolgus PVRIG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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