Cynomolgus PVRIG Protein (PVR-CM401)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus PVRIG, hFc Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PVRIG Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Thr41-Asp171

Catalog: PVR-CM401

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Product Description	Recombinant Cynomolgus PVRIG Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Thr41-Asp171.
Product Background	PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.
Product Category	Recombinant Protein

Target	PVRIG
Synonyms	C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1
Gene/Protein ID	-A0A2K5WVV8-1
Species	Cynomolgus
Exact Sequence	Thr41-Asp171
Molecular Weight	The protein has a predicted MW of 40.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus PVRIG, hFc Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PVRIG Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus PVRIG, hFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-2, His Tag with an affinity constant of 0.23 uM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus PVRIG, hFc Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PVRIG Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).
	The purity of Cynomolgus PVRIG is greater than 95% as determined by SEC-HPLC.
	Cynomolgus PVRIG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.