• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Cynomolgus RGM-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 1.7ng/ml determined by ELISA.
  • Amino Acid Range: Gln36-Asp401
Catalog #RGM-CM10C
Price
Regular price $346.50
Regular price Sale price $346.50
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Cynomolgus RGM-C Protein (RGM-CM10C) Summary

Product Description

 Recombinant Cynomolgus RGM-C Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln36-Asp401.

Product Background

 RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Cynomolgus RGM-C Protein (RGM-CM10C) Specifications/Details

Protein
 RGM-C
Synonyms
 Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Gln36-Asp401
Molecular Weight
 The protein has a predicted MW of 39.93 kDa. Due to autocatalytic cleavage, the protein migrates to 52-62 kDa (mature), 30-40 kDa (C-terminus chain) and 20-25 kDa (N-terminus chain) based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus RGM-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 1.7ng/ml determined by ELISA.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus RGM-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 1.7ng/ml determined by ELISA. Immobilized Cynomolgus RGM-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 1.7ng/ml determined by ELISA.
Cynomolgus RGM-C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus RGM-C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus RGM-C is greater than 90% as determined by SEC-HPLC. The purity of Cynomolgus RGM-C is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...