- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Cynomolgus/Rhesus macaque B7-H4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Phe29-Ala258
Product Description |
Recombinant Cynomolgus/Rhesus macaque B7-H4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe29-Ala258. |
Product Background |
B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status-. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Protein |
B7-H4 |
Synonyms |
B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291 |
Accession |
|
Species |
Cynomolgus/Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Phe29-Ala258 |
Molecular Weight |
The protein has a predicted MW of 26.41 kDa. Due to glycosylation, the protein migrates to 48-63 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus/Rhesus macaque B7-H4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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