Product Description |
Recombinant Cynomolgus/Rhesus macaque B7-H5/Gi24/VISTA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe33-Thr192. |
Product Background |
B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. |
Product Category |
Recombinant Protein |
Target |
B7-H5/Gi24/VISTA |
Synonyms |
B7H5; B7-H5; VISTA; VSIR ; PD1H; PD-1H; 4632428N05Rik; C10orf54; Dies1; Gi24; PP2135; SISP1 |
Accession |
|
Species |
Cynomolgus/Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Phe33-Thr192 |
Molecular Weight |
The protein has a predicted MW of 19.13 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus/Rhesus macaque B7-H5, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Cynomolgus/Rhesus macaque B7-H5, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). |
|
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Cynomolgus/Rhesus macaque B7-H5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus/Rhesus macaque B7-H5 is greater than 95% as determined by SEC-HPLC. |
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