Cynomolgus/Rhesus macaque CD19 Protein (CD1-CM129)

Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus/Rhesus macaque CD19, His Tag with an affinity constant of 0.26 uM as determined in SPR assay (Biacore T200).

Immobilized Cynomolgus/Rhesus macaque CD19, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD19 Antibody, hFc Tag with the EC50 of 1.16ug/ml determined by ELISA.

Cynomolgus/Rhesus macaque CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Anti-CD19 Antibody, hFc Tag on ProA-Biosensor can bind Cynomolgus/Rhesus macaque CD19, His Tag with an affinity constant of 0.43 µM as determined in BLI assay .

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Cynomolgus/Rhesus macaque CD19, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD19 Antibody, hFc Tag with the EC50 of 1.16ug/ml determined by ELISA. The affinity constant of 0.26 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gln21-Lys292

Catalog #CD1-CM129

Price

$413⁰⁰

Size

Order by 4pm ET for delivery on:

Cynomolgus/Rhesus macaque CD19 Protein (CD1-CM129) Summary

Product Description

Recombinant Cynomolgus/Rhesus macaque CD19 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln21-Lys292.

Product Background

CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca²⁺ stores.

Cynomolgus/Rhesus macaque CD19 Protein (CD1-CM129) Specifications/Details

Protein	CD19
Synonyms	CD19; B4; CVID3; Leu-12; MGC12802;Leu12
Accession	A0A2K5W8L9(Cynomolgus) / F7F486(Rhesus macaque)
Species	Cynomolgus/Rhesus macaque
Biotinylated	no
Amino Acid Range	Gln21-Lys292
Molecular Weight	The protein has a predicted MW of 34.0 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus/Rhesus macaque CD19, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD19 Antibody, hFc Tag with the EC50 of 1.16ug/ml determined by ELISA. The affinity constant of 0.26 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus/Rhesus macaque CD19, His Tag with an affinity constant of 0.26 uM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus/Rhesus macaque CD19, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD19 Antibody, hFc Tag with the EC50 of 1.16ug/ml determined by ELISA.
	Cynomolgus/Rhesus macaque CD19 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-CD19 Antibody, hFc Tag on ProA-Biosensor can bind Cynomolgus/Rhesus macaque CD19, His Tag with an affinity constant of 0.43 µM as determined in BLI assay .