Cynomolgus/Rhesus macaque DKK1-Protein (DKK-CM301)
Cynomolgus/Rhesus macaque DKK1-Protein (DKK-CM301)
Cynomolgus/Rhesus macaque DKK1-Protein (DKK-CM301)
Cynomolgus/Rhesus macaque DKK1-Protein (DKK-CM301)
  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus/Rhesus macaque DKK1, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Thr32-His266
Catalog #DKK-CM301
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Product Description
 Recombinant Cynomolgus/Rhesus macaque DKK1-Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Thr32-His266.
Product Background
 Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Product Category
 Recombinant Protein
Target
 DKK1
Accession
Species
 Cynomolgus/Rhesus macaque
Biotinylated
 no
Amino Acid Range
 Thr32-His266
Molecular Weight
 The protein has a predicted MW of 51.42 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus/Rhesus macaque DKK1, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-mFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus/Rhesus macaque DKK1, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test).
Cynomolgus/Rhesus macaque DKK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus/Rhesus macaque DKK1 is greater than 95% as determined by SEC-HPLC.
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