Name: Cynomolgus/Rhesus macaque DKK1-Protein, Ultra Low Endotoxin (DKK-CM301-UL)
Brand: KactusBio
SKU: DKK-CM301-UL-100UG
Price: 327.0 USD
Availability: InStock

Cynomolgus/Rhesus macaque DKK1-Protein, Ultra Low Endotoxin (DKK-CM301-UL)

Immobilized Cynomolgus/Rhesus macaque DKK1, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA (QC Test).

Cynomolgus/Rhesus macaque DKK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus/Rhesus macaque DKK1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-mFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus/Rhesus macaque DKK1, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Thr32-His266

Catalog #DKK-CM301-UL

Size

100UG

500UG

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Product Description	Recombinant Cynomolgus/Rhesus macaque DKK1-Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Thr32-His266.
Product Background	Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	DKK1
Synonyms	Dickkopf-1; Dkk1; SK
Accession	XP_005565907.1(Cynomolgus) / NP_001247454.1(Rhesus macaque)
Species	Cynomolgus/Rhesus macaque
Biotinylated	no
Amino Acid Range	Thr32-His266
Molecular Weight	The protein has a predicted MW of 51.42 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus/Rhesus macaque DKK1, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-mFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Cynomolgus/Rhesus macaque DKK1, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA (QC Test).
	Cynomolgus/Rhesus macaque DKK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Cynomolgus/Rhesus macaque DKK1 is greater than 95% as determined by SEC-HPLC.