Regular price
$346.50
Order by 4pm ET for delivery on:
Cynomolgus/Rhesus macaque FcRn Protein (FRN-CM101) Summary
Product Description |
Recombinant Cynomolgus/Rhesus macaque FcRn Protein is expressed from HEK293 with His tag at the C-Terminus of FCGRT.It contains Ala24-Ser297 (FCGRT) and Ile21-Met119 (B2M). |
Product Background |
The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Cynomolgus/Rhesus macaque FcRn Protein (FRN-CM101) Specifications/Details
Protein |
FcRn |
Synonyms |
FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M |
Accession |
|
Species |
Cynomolgus/Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Ala24-Ser297(FCGRT) & Ile21-Met119(B2M) |
Molecular Weight |
The protein has a predicted MW of 31.5 kDa (FCGRT) and 11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT) and 13-15 kDa (B2M) based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM PB, 250mM NaC (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Cynomolgus/Rhesus macaque FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.18 uM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA (QC Test). |
|
|
Cynomolgus/Rhesus macaque FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Cynomolgus/Rhesus macaque FcRn is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
