Name: Cynomolgus/Rhesus macaque FcRn Protein (FRN-CM101)
Brand: KactusBio
SKU: FRN-CM101-100UG
Price: 346.5 USD
Availability: InStock

SPR sensorgram showing binding kinetics of multiple concentrations of an analyte to a ligand, highlighting real-time molecular interaction on a Biacore system.

Cynomolgus/Rhesus macaque FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.18 uM as determined in SPR assay (Biacore T200).

Cynomolgus Serum Albumin, His Tag immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque FcRn, His Tag with an affinity constant of 0.13 uM as determined in SPR assay (Biacore T200).

Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA (QC Test).

Cynomolgus/Rhesus macaque FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus/Rhesus macaque FcRn is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)

Catalog #FRN-CM101

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Cynomolgus/Rhesus macaque FcRn Protein (FRN-CM101) Summary

Product Description	Recombinant Cynomolgus/Rhesus macaque FcRn Protein is expressed from HEK293 with His tag at the C-Terminus of FCGRT.It contains Ala24-Ser297 (FCGRT) and Ile21-Met119 (B2M).
Product Background	The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.
Product Category	Recombinant Protein / Antibody discovery / Fc receptors

Cynomolgus/Rhesus macaque FcRn Protein (FRN-CM101) Specifications/Details

Protein	FcRn
Synonyms	FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
Accession	Q8SPV9(FCGRT)&Q8SPW0(B2M)
Species	Cynomolgus/Rhesus macaque
Biotinylated	no
Amino Acid Range	Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)
Molecular Weight	The protein has a predicted MW of 31.5 kDa (FCGRT) and 11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT) and 13-15 kDa (B2M) based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM PB, 250mM NaC (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Cynomolgus/Rhesus macaque FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.18 uM as determined in SPR assay (Biacore T200).
	Cynomolgus Serum Albumin, His Tag immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque FcRn, His Tag with an affinity constant of 0.13 uM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA (QC Test).
	Cynomolgus/Rhesus macaque FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Cynomolgus/Rhesus macaque FcRn is greater than 95% as determined by SEC-HPLC.