Cynomolgus/Rhesus macaque FcRn Protein (FRN-CM101)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. The affinity constant of 5.46nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala24-Ser297 (FCGRT)&Ile21-Met119 (B2M)

Catalog: FRN-CM101

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Product Description Recombinant Cynomolgus/Rhesus macaque FcRn Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Ala24-Ser297 (FCGRT)&Ile21-Met119 (B2M).
Product Category Recombinant Protein
Target FcRn
Synonyms FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
Gene/Protein ID Q8SPV9 (FCGRT)&Q8SPW0 (B2M)
Species Cynomolgus/Rhesus macaque
Exact Sequence Ala24-Ser297 (FCGRT)&Ile21-Met119 (B2M)
Molecular Weight The protein has a predicted MW of 31.5 kDa (FCGRT)&11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT)&13-15 kDa (B2M) based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. The affinity constant of 5.46nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Liquid
Shipping Shipped with blue ice or dry ice.
Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus/Rhesus macaque FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-HER2 Antibody with an affinity constant of 5.46nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA.
The purity of Cynomolgus/Rhesus macaque FcRn is greater than 95% as determined by SEC-HPLC.
Cynomolgus/Rhesus macaque FcRn on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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