Cynomolgus/Rhesus macaque FcRn Protein (FRN-CM101)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2_g/ml (100_l/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. The affinity constant of 0.18 _M as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)

Catalog: FRN-CM101

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Product Description Recombinant Cynomolgus/Rhesus macaque FcRn Protein is expressed from Expi293 with His tag at the C-terminal.It contains Ala24-Ser297 (FCGRT)&Ile21-Met119 (B2M).
Product Category Recombinant Protein
Target FcRn
Synonyms FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
Gene/Protein ID Q8SPV9(FCGRT)&Q8SPW0(B2M)
Species Cynomolgus/Rhesus macaque
Exact Sequence Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)
Molecular Weight The protein has a predicted MW of 31.5 kDa (FCGRT)&11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT)&13-15 kDa (B2M) based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2_g/ml (100_l/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. The affinity constant of 0.18 _M as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus/Rhesus macaque FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-HER2 Antibody with an affinity constant of 0.18 _M as determined in SPR assay (Biacore T200).
Cynomolgus Serum Albumin, His Tag immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque FcRn, His Tag with an affinity constant of 0.108 _M as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2_g/ml (100_l/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA.
The purity of Cynomolgus/Rhesus macaque FcRn is greater than 95% as determined by SEC-HPLC.
Cynomolgus/Rhesus macaque FcRn on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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