Cynomolgus/Rhesus macaque FcRn Protein (FRN-CM101)
Catalog: FRN-CM101
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Product Description | Recombinant Cynomolgus/Rhesus macaque FcRn Protein is expressed from Expi293 with His tag at the C-terminal.It contains Ala24-Ser297 (FCGRT)&Ile21-Met119 (B2M). |
Product Category | Recombinant Protein |
Target | FcRn |
Synonyms | FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M |
Gene/Protein ID | Q8SPV9(FCGRT)&Q8SPW0(B2M) |
Species | Cynomolgus/Rhesus macaque |
Exact Sequence | Ala24-Ser297(FCGRT) & Ile21-Met119(B2M) |
Molecular Weight | The protein has a predicted MW of 31.5 kDa (FCGRT)&11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT)&13-15 kDa (B2M) based on Tris-Bis PAGE result. |
Activity | Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. The affinity constant of 0.18 μM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | C-His |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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