Cynomolgus/Rhesus macaque FcRn Protein (FRN-CM101)
Catalog: FRN-CM101
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| Product Description | Recombinant Cynomolgus/Rhesus macaque FcRn Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala24-Ser297 (FCGRT)&Ile21-Met119 (B2M). |
Product Background |
The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer. |
Product Category |
Recombinant Protein |
Target |
FcRn |
| Synonyms | FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M |
Gene/Protein ID |
Q8SPV9(FCGRT)&Q8SPW0(B2M) |
Species |
Cynomolgus/Rhesus macaque |
Exact Sequence |
Ala24-Ser297(FCGRT) & Ile21-Met119(B2M) |
| Molecular Weight | The protein has a predicted MW of 31.5 kDa (FCGRT)&11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT)&13-15 kDa (B2M) based on Tris-Bis PAGE result. |
Activity |
Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Cynomolgus/Rhesus macaque FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.18 uM as determined in SPR assay (Biacore T200). |
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Cynomolgus Serum Albumin, His Tag immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque FcRn, His Tag with an affinity constant of 0.108 uM as determined in SPR assay (Biacore T200). |
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Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test). |
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The purity of Cynomolgus/Rhesus macaque FcRn is greater than 95% as determined by SEC-HPLC. |
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Cynomolgus/Rhesus macaque FcRn on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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