Cynomolgus/Rhesus macaque FcRn Protein (FRN-CM101)
Catalog: FRN-CM101
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| Product Description | Recombinant Cynomolgus/Rhesus macaque FcRn Protein is expressed from Expi293 with His tag at the C-terminal.It contains Ala24-Ser297 (FCGRT)&Ile21-Met119 (B2M). |
| Product Category | Recombinant Protein |
| Target | FcRn |
| Synonyms | FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M |
| Gene/Protein ID | Q8SPV9(FCGRT)&Q8SPW0(B2M) |
| Species | Cynomolgus/Rhesus macaque |
| Exact Sequence | Ala24-Ser297(FCGRT) & Ile21-Met119(B2M) |
| Molecular Weight | The protein has a predicted MW of 31.5 kDa (FCGRT)&11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT)&13-15 kDa (B2M) based on Tris-Bis PAGE result. |
| Activity | Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2_g/ml (100_l/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. The affinity constant of 0.18 _M as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per _g by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Cynomolgus/Rhesus macaque FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-HER2 Antibody with an affinity constant of 0.18 _M as determined in SPR assay (Biacore T200). |
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Cynomolgus Serum Albumin, His Tag immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque FcRn, His Tag with an affinity constant of 0.108 _M as determined in SPR assay (Biacore T200). |
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Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2_g/ml (100_l/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. |
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The purity of Cynomolgus/Rhesus macaque FcRn is greater than 95% as determined by SEC-HPLC. |
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Cynomolgus/Rhesus macaque FcRn on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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