Cynomolgus/Rhesus macaque OX40 Ligand/TNFSF4 Protein (OXL-CM140)
Cynomolgus/Rhesus macaque OX40 Ligand/TNFSF4 Protein (OXL-CM140)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln81-Leu213
Catalog #OXL-CM140
Regular price $315.00
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Product Description
Recombinant Cynomolgus/Rhesus macaque OX40 Ligand/TNFSF4 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Gln81-Leu213.
Product Background
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.
Product Category
Recombinant Protein
Target
OX40 Ligand/TNFSF4
Accession
Species
Cynomolgus/Rhesus macaque
Biotinylated
no
Amino Acid Range
Gln81-Leu213
Molecular Weight
The protein has a predicted MW of 17.01 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA.
Cynomolgus/Rhesus macaque OX40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus/Rhesus macaque OX40 Ligand is greater than 95% as determined by SEC-HPLC.
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