ELISA dose-response curve showing binding interaction between immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag, and human OX40, hFc Tag.
Cynomolgus/Rhesus macaque OX40 Ligand/TNFSF4 Protein (active trimer) (OXL-CM140)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA.
  • Amino Acid Range: Gln81-Leu213 (Cynomolgus) / Gln51-Leu183 (Rhesus macaque)
Catalog #OXL-CM140
Regular price $346.50
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Cynomolgus/Rhesus macaque OX40 Ligand/TNFSF4 Protein (active trimer) (OXL-CM140) Summary

Product Description

Recombinant Cynomolgus/Rhesus macaque OX40 Ligand/TNFSF4 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Gln81-Leu213 (Cynomolgus) / Gln51-Leu183 (Rhesus macaque).

Product Background

Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Cynomolgus/Rhesus macaque OX40 Ligand/TNFSF4 Protein (active trimer) (OXL-CM140) Specifications/Details

Protein
OX40 Ligand/TNFSF4
Synonyms
OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L;-TXGP1; Glycoprotein Gp34
Accession
Species
Cynomolgus/Rhesus macaque
Biotinylated
no
Amino Acid Range
Gln81-Leu213 (Cynomolgus) / Gln51-Leu183 (Rhesus macaque)
Molecular Weight
The protein has a predicted MW of 17.01 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA.
Product Tag
N-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA.
Cynomolgus/Rhesus macaque OX40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus/Rhesus macaque OX40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus/Rhesus macaque OX40 Ligand is greater than 95% and the molecular weight of this protein is around 60-70 kDa as determined by SEC-MALS. The purity of Cynomolgus/Rhesus macaque OX40 Ligand is greater than 95% and the molecular weight of this protein is around 60-70 kDa as determined by SEC-MALS.
The purity of Cynomolgus/Rhesus macaque OX40 Ligand is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus/Rhesus macaque OX40 Ligand is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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