Product Description |
Recombinant Cynomolgus/Rhesus macaque OX40 Ligand/TNFSF4 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Gln81-Leu213 (Cynomolgus) / Gln51-Leu183 (Rhesus macaque). |
Product Background |
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. |
Product Category |
Recombinant Protein |
Target |
OX40 Ligand/TNFSF4 |
Synonyms |
OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L;-TXGP1; Glycoprotein Gp34 |
Accession |
|
Species |
Cynomolgus/Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Gln81-Leu213 (Cynomolgus) / Gln51-Leu183 (Rhesus macaque) |
Molecular Weight |
The protein has a predicted MW of 17.01 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. |
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Cynomolgus/Rhesus macaque OX40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus/Rhesus macaque OX40 Ligand is greater than 95% as determined by SEC-HPLC. |
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