• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus/Rhesus macaque OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.
  • Amino Acid Range: Lys28-Ala214
Catalog #OX4-CM140
Price
Regular price $22330
Regular price Sale price $22330
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Size
Avantor VWR Fisher Scientific

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Cynomolgus/Rhesus macaque OX40/TNFRSF4/CD134 Protein (OX4-CM140) Summary

Product Description

 Recombinant Cynomolgus/Rhesus macaque OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Lys28-Ala214.

Product Background

 Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.

Cynomolgus/Rhesus macaque OX40/TNFRSF4/CD134 Protein (OX4-CM140) Specifications/Details

Protein
 OX40/TNFRSF4/CD134
Synonyms
 CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue;-TXGP1L; IMD16;ACT135; Ly70
Accession
Species
 Cynomolgus/Rhesus macaque
Biotinylated
 no
Amino Acid Range
 Lys28-Ala214
Molecular Weight
 The protein has a predicted MW of 21.84 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus/Rhesus macaque OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus/Rhesus macaque OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA. Immobilized Cynomolgus/Rhesus macaque OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.
Cynomolgus/Rhesus macaque OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus/Rhesus macaque OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus/Rhesus macaque OX40 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus/Rhesus macaque OX40 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...