Cynomolgus Semaphorin 4D/SEMA4D/CD100 Protein (SEM-CM14D)
Cynomolgus Semaphorin 4D/SEMA4D/CD100 Protein (SEM-CM14D)
Cynomolgus Semaphorin 4D/SEMA4D/CD100 Protein (SEM-CM14D)
Cynomolgus Semaphorin 4D/SEMA4D/CD100 Protein (SEM-CM14D)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus Semaphorin 4D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe24-Arg734
Catalog #SEM-CM14D
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Product Description
 Recombinant Cynomolgus Semaphorin 4D/SEMA4D/CD100 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe24-Arg734.
Product Background
 Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.
Product Category
 Recombinant Protein
Target
 Semaphorin 4D/SEMA4D/CD100
Synonyms
 Semaphorin-4D; CD100; SEMA4D; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Phe24-Arg734
Molecular Weight
 The protein has a predicted MW of 80.1 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus Semaphorin 4D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus Semaphorin 4D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 11.8ng/ml determined by ELISA.
Cynomolgus Semaphorin 4D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus Semaphorin 4D is greater than 95% as determined by SEC-HPLC.

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