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Cynomolgus SERPINF2/A2AP Protein (SPF-CM101) Summary
Product Description |
Recombinant Cynomolgus SERPINF2/A2AP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met27-Lys490. |
Product Background |
Large-pool solvent/detergent (SD) plasma for transfusion exhibits reduced alpha 2-antiplasmin (alpha2-AP; SERPINF2) functional activity. The reason for the loss of alpha2-AP has not been described and could be due to the SD incubation itself and/or to the processing steps implemented to remove the solvent and the detergent. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Cynomolgus SERPINF2/A2AP Protein (SPF-CM101) Specifications/Details
Protein |
SERPINF2/A2AP |
Synonyms |
Alpha-2-AP; AAP; PLI; SERPINF2; A2AP; ALPHA-2-PI; API; Serpin F2 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Met27-Lys490 |
Molecular Weight |
The protein has a predicted MW of 52.83 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus SERPINF2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SERPINF2 Antibody, hFc Tag with the EC50 of 53.5ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Cynomolgus SERPINF2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SERPINF2 Antibody, hFc Tag with the EC50 of 53.5ng/ml determined by ELISA. |
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Cynomolgus SERPINF2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus SERPINF2 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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