- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: The affinity constant of 0.13 uM as determined in SPR assay (Biacore T200). See testing image for detail.
- Amino Acid Range: Asp25-Ala608
Product Description |
Recombinant Cynomolgus Serum Albumin Protein is expressed from HEK293 with His tag at the C-Terminus.; It contains Asp25-Ala608. |
Product Background |
Albumins are multifunctional proteins present in the blood serum of animals. They can bind and transport a wide variety of ligands which they accommodate due to their conformational flexibility. Serum albumins are highly conserved both in amino acid sequence and three-dimensional structure. Several mammalian and avian serum albumins (SAs) are also allergens. Sensitization to one of the SAs coupled with the high degree of conservation between SAs may result in cross-reactive antibodies in allergic individuals. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
Serum Albumin |
Synonyms |
Albumin; ALB; Serum albumin; ANALBA; FDAH; PRO0883; PRO0903; PRO1341 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Asp25-Ala608 |
Molecular Weight |
The protein has a predicted MW of 67.1 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.13 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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